Attachment of biomolecules (protein and DNA) to amino-functionalized carbon nanotubes
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摘要: 报告－种氨基功能化处理的多壁碳纳米管（f-MWCNTs）用于生物分子〔如牛血浆蛋白素（BSA）蛋白质及脱氧核醣核酸（DNA）〕的接枝处理高效方法。以苯—二茂铁喷入氩氛炉于～850 ℃裂解制得MWCNTs,，而后向纳米管表面导入羧基化合物，再以氨基基团对MWCNTs,进行功能化处理。该过程包括：乙二胺与羧基基团直接结合，经酰胺化引入胺基基团。对制得的MWCNTs、 f-MWCNTs以及由BSA蛋白质和DNA接枝的氨化f-MWCNTs等样品，采用SEM、TEM、FTIR进行表征。结果表明：生物分子（BSA蛋白质和DNA）接枝于氨化的f-MWCNTs。Abstract: An efficient method for the attachment of biomolecules [e.g. bovine serum albumin (BSA) protein and deoxyribonucleic acid (DNA)] to amino-group-functionalized multiwalled carbon nanotubes (f-MWCNTs) was reported. MWCNTs were prepared by spray pyrolysis of a benzene-ferrocene solution in argon atmosphere at ~850°C followed by functionalization with an amino group by chemical modification of carboxylic groups introduced on the nanotube surface. This process involves a direct coupling of ethylenediamine with carboxylic groups to introduce amino groups by amide formation. The as-synthesized MWCNTs, f-MWCNTs, and amino f- MWCNTs with BSA protein and DNA were characterized by scanning and transmission electron microscopy, and Fourier transform infrared spectroscopy, which confirm the attachment of biomolecules (BSA protein and DNA) to the amino f-MWCNTs.
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Key words:
- Carbon nanotubes /
- Functionalization /
- Protein /
- DNA
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