水溶性碳纳米管对人胚肾和肝癌细胞毒性的研究

于世平; 苏旭东; 杜晶磊; 王军丽; 高宇端; 张利; 陈琳; 杨永珍; 刘旭光

doi:10.1016/S1872-5805(18)60325-7

水溶性碳纳米管对人胚肾和肝癌细胞毒性的研究

doi: 10.1016/S1872-5805(18)60325-7

于世平^1,2,
苏旭东³,
杜晶磊¹,
王军丽^2,4,
高宇端⁵,
张利⁶,
陈琳^2,4,
杨永珍^2,4,,
刘旭光²

1.
山西医科大学第二医院介入治疗科, 山西太原 030001;
2.
太原理工大学新材料界面与工程教育部重点实验室, 山西太原 030024;
3.
山西大医院血管外科, 山西太原 030032;
4.
太原理工大学新材料工程技术研究中心, 山西太原 030024;
5.
山西大医院眼科, 山西太原 030032;
6.
山西大医院泌尿外科, 山西太原 030032

基金项目: 山西省重点研发计划（201703D321015-1，201603D111010）；国家自然科学基金（U1610255，U1607120）；山西省科技创新重点团队（2015013002-10，201605D131045-10）.

详细信息

作者简介:
于世平,博士,副教授.E-mail:yushiping6@126.com

通讯作者:
杨永珍,博士,教授.E-mail:yyztyut@126.com

中图分类号: R34
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- 被引次数: 0
出版历程
- 收稿日期: 2017-10-12
- 录用日期: 2018-02-11
- 修回日期: 2018-01-22
- 刊出日期: 2018-02-28

The cytotoxicity of water-soluble carbon nanotubes on human embryonic kidney and liver cancer cells

1.
Interventional Treatment Department, Second Hospital of Shanxi Medical University, Taiyuan 030001, China;
2.
Key Laboratory of Interface Science and Engineering in Advanced Materials of Ministry of Education, Taiyuan University of Technology, Taiyuan 030024, China;
3.
Vascular Surgery Department, Shanxi Dayi Hospital, Taiyuan 030032, China;
4.
Research Center on Advanced Materials Science and Technology, Taiyuan University of Technology, Taiyuan 030024, China;
5.
Ophthalmology Department, Shanxi Dayi Hospital, Taiyuan 030032, China;
6.
Urology Department, Shanxi Dayi Hospital, Taiyuan 030032, China

Funds: Shanxi Provincial Key Research and Development Program (201703D321015-1, 201603D111010);National Natural Science Foundation of China (U1610255, U1607120);Shanxi Provincial Key Innovative Research Team in Science and Technology (2015013002-10, 201605D131045-10).

摘要

摘要: 研究了聚乙二醇改性碳纳米管（PEG-CNTs）对人胚肾细胞（293T）及人肝癌细胞（HepG₂）的体外细胞毒性。在染毒24、48和72 h后，用水溶性四氮唑法检测不同浓度PEG-CNTs对细胞活性的影响；采用碘化丙啶染色研究不同浓度的PEG-CNTs在对HepG₂细胞染毒24 h后死亡细胞染色情况，并用流式细胞术定量测定细胞死亡率。结果表明：水溶性较好的PEG-CNTs作用于293T细胞与HepG₂细胞，其毒性存在浓度依赖关系，毒性会随着浓度的升高而增大；根据ISO2109932-5细胞毒性标准，浓度≤ 100 μg/mL时为毒性Ⅰ级，认为PEG-CNTs没有毒性；当浓度为200 μg/mL时，毒性变为Ⅱ级，为轻微毒性，说明低剂量的PEG-CNTs对细胞未显示毒性作用；在研究的时间范围内未发现随时间延长而毒性等级的变化。
- 水溶性碳纳米管 /
- 人胚肾细胞 /
- 肝癌细胞 /
- 细胞毒性
Abstract: The cytotoxicity of polyethylene glycol-modified carbon nanotubes (PEG-CNTs) on human embryonic kidney cells (293T) and hepatoma cells (HepG₂) was studied in vitro. The effect of the concentration of PEG-CNTs on the cell activity and survival rate was detected by a colorimetric assay method after exposure for 24, 48 and 72 h. The dead HepG₂ cells obtained by incubation in solutions with different concentrations of PEG-CNTs for 24 h can be stained by a dye, propidium iodide, and the cell mortality was determined by flow cytometry. Results show that the toxicity of PEG-CNTs with a good water solubility towards 293T and HepG₂ cells increases with increasing PEG-CNT concentration. When the concentration of the PEG-CNTs is less than 100 μg/mL, the toxicity of the PEG-CNTs is Grade Ⅰ(non-toxic) and when it is greater than 100 μg/mL but less than 200 μg/mL it is Grade Ⅱ(mildly toxic), according to the ISO2109932-5 cytotoxicity standard. The toxicity grade does not change with increased time.
- Water-soluble carbon nanotubes /
- Human embryonic kidney cells /
- Hepatoma cell /
- Cytotoxicity

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