生物相溶性多孔炭-香豆素复合材料的制备及其细胞成像和靶向给药

S. Yallappa; Shoriya Aruni Abdul Manaf; Gurumurthy Hegde

doi:10.1016/S1872-5805(18)60332-4

生物相溶性多孔炭-香豆素复合材料的制备及其细胞成像和靶向给药

doi: 10.1016/S1872-5805(18)60332-4

1.
BMS R and D Centre, BMS College of Engineering, Bangalore560019, Karnataka, India;
2.
Department of Chemistry, Gokhale Centenary College, Ankola, Uttara Kannada581314, India;
3.
Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang26300, Gambang, Kuantan, Malaysia

详细信息

通讯作者:
Gurumurthy Hegde.E-mail:murthyhegde@gmail.com

中图分类号: TB332
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出版历程
- 收稿日期: 2017-12-30
- 录用日期: 2018-04-28
- 修回日期: 2018-04-02
- 刊出日期: 2018-04-28

Synthesis of a biocompatible nanoporous carbon and its conjugation with florescent dye for cellular imaging and targeted drug delivery to cancer cells

1.
BMS R and D Centre, BMS College of Engineering, Bangalore 560019, Karnataka, India;
2.
Department of Chemistry, Gokhale Centenary College, Ankola, Uttara Kannada 581314, India;
3.
Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang 26300, Gambang, Kuantan, Malaysia

摘要

摘要: 将棕榈叶在惰性气氛中经一步热解过程制备出多孔炭（NPCs），并结合香豆素-6（C-6）形成NPCs-C-6复合荧光染料来进行癌细胞成像及靶向给药。通过XRD和Raman分析可知，NPCs具有高的含碳量。傅里叶红外分析表明棕榈叶中含有纤维素、半纤维素和木质素，这有利于NPCs的形成，以及香豆素-6为导航分子的细胞成像和靶向给药。采用SEM和TEM观察NPCs结合前后的结构形貌，XPS表征元素组成。采用Zeta电位为初始指标来确定颗粒分散质量，以适合生物医学应用。NPCs-C-6分析细胞毒性和成像试验表明，对正常细胞系（MDCK：ATCC® CCL-34^TM）有高的生物相溶性，对人体癌细胞系（A-375：ATCC® CRL-1619^TM与N2A：ATCC® CCL-131^TM）有高的毒性。因此，这些研究结果有望为生物基功能NPCs作治疗载体的研究进展提供新思路。
- 多孔炭 /
- 香豆素-6 /
- 细胞成像 /
- 靶向给药
Abstract: A nanoporous carbon (NPC) was synthesized from Oil palm leaves (OPL) by pyrolysis at 600℃ in a N₂ atmosphere, oxidized by a mixture of sulfuric and nitric acids (3:1 vol/vol) and conjugated with coumarin-6 as a fluorescence dye for cellular imaging and drug delivery to cancer cells. The structure, morphology and dispersion stability of NPC in aqueous media before and after the conjugation were investigated by XRD, FT-IR, Raman spectroscopy, SEM, TEM and zeta potential measurement. Cell uptake for the conjugated NPC was investigated by fluorescence microscopy. Results indicate that the cellulose, hemicelluloses and lignin in OPL convert to a graphitic structure by pyrolysis. NPC consists of spherical nanoparticles with diameters of 30-50 nm and has a high graphitic content with an I_D/I_G ratio of 0.7. The coumarin-6 is successfully conjugated to NPC by forming a complex. Both NPC and the conjugated NPC have a high dispersion stability in aqueous media. NPC is biocompatible with a negligible cytotoxicity. The conjugated NPC exhibits a high cell uptake, is highly biocompatible for normal cells and toxic for tested human cancer cells. NPC is a good candidate for cellular imaging and targeted drug delivery.
- Nanoporous carbons /
- Coumarin-6 /
- Cellular imaging /
- Targeted drug delivery

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