Preparation of nitrogen-doped carbon dots with a high fluorescence quantum yield for the highly sensitive detection of Cu2+ ions, drawing anti-counterfeit patterns and imaging live cells
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摘要: 随着科学技术的进步，荧光碳质材料越来越多受到人们的关注。具有高荧光量子产率碳点的研制对其在荧光传感等应用具有重要意义。本文以柠檬酸铵和三乙基四胺为原料，采用一步微波法制备了一种低成本、绿色环保、荧光量子产率高（29.83%）的水溶性荧光碳点（N-CDs），并对N-CDs的结构和光学性能进行了表征。N-CDs具有高荧光、良好的生物相容性和光学稳定性。与其它已报道的碳点纳米材料相比，N-CDs具有更加突出的光学性质以及对Cu2+高选择、高敏感的检测，即合成的N-CDs的荧光强度能够被Cu2+猝灭且在0.01~11 μM存在良好的线性，对Cu2+的检出限为4.5 nM （S/N=3），进一步应用到实际水样中Cu2+含量的测定。此外，合成的N-CDs还可用于绘制荧光图案。最后，将强发光、低毒的N-CDs应用于活细胞（SMMC-7721）的荧光图像和细胞中监测Cu2+的存在，表明合成的N-CDs具有广阔的应用前景。Abstract: Water-soluble luminescent nitrogen-doped CDs (N-CDs) having a high QY of 29.83% were prepared by a one-step microwave-assisted pyrolysis method using ammonium citrate and triethylenetetramine as the precursors. The microstructure, optical properties and biocompatibility of the N-CDs were investigated. Results indicate that they exhibit a strong fluorescence, favorable biocompatibility and excellent optical stability. Their photoluminescence is quenched by Cu2+ ions through the formation of a complex between the N-CDs and Cu2+, which can be used for Cu2+ detection. Compared with other CD-based nanomaterials, the N-CDs show outstanding optical properties, and excellent selectivity and sensitivity for Cu2+ detection with a linear range of 0.01-11 μM and a detection limit of 4.5 nM. The selectivity for Cu2+ detection is so good that the N-CDs have been successfully used in Cu2+ detection for real water samples. They have also been used to draw fluorescent patterns and image living cells.
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Key words:
- Carbon dots /
- Highly fluorescence quantum yield /
- Cu2+ sensor /
- Fluorescent patterns /
- Cells imaging
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