Preparation and properties of a glucose biosensor electrode based on an ionic liquid-functionalized graphene/carbon nanotube composite

ZOU Lei; WANG Shan-shan; QIU Jun

doi:10.1016/S1872-5805(20)60472-3

Volume 35 Issue 1

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ZOU Lei, WANG Shan-shan, QIU Jun. Preparation and properties of a glucose biosensor electrode based on an ionic liquid-functionalized graphene/carbon nanotube composite. New Carbon Mater., 2020, 35(1): 12-19. doi: 10.1016/S1872-5805(20)60472-3

Citation:

ZOU Lei, WANG Shan-shan, QIU Jun. Preparation and properties of a glucose biosensor electrode based on an ionic liquid-functionalized graphene/carbon nanotube composite. New Carbon Mater., 2020, 35(1): 12-19. doi: 10.1016/S1872-5805(20)60472-3

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Preparation and properties of a glucose biosensor electrode based on an ionic liquid-functionalized graphene/carbon nanotube composite

doi: 10.1016/S1872-5805(20)60472-3

1.
School of Materials Science and Engineering, Tongji University, Shanghai 201804, China;
2.
Key Laboratory of Advanced Civil Engineering Materials(Tongji University), Education of Ministry, Shanghai 201804, China

Funds: Key Basic Research Projects of Science and Technology Commission of Shanghai(13JC1405300).

Received Date: 2019-09-30
Accepted Date: 2020-04-02
Rev Recd Date: 2020-01-15
Publish Date: 2020-02-29

Abstract

Abstract

A novel glucose biosensor electrode was fabricated by loading a glassy carbon electrode with a 1-methyl imidazole-based ionic liquid-functionalized graphene/carbon nanotube (CNTs) composite, which was used to immobilize horseradish peroxidase (HRP) and glucose oxidase (GOD) for glucose detection. SEM, AFM and FTIR were used to investigate the microstructures and morphology of the electrode. The electrochemical performance of the electrode was investigated by cyclic voltammetry and electrochemical impedance spectroscopy. Results indicate that the electrode has an excellent electrocatalytic activity towards glucose. A biosensor prepared at the optimal loading amount of the composite and HRP/GOD ratio has a linear range of 0.004-5 mM, a detection limit of 3.99×10^-7 M and a sensitivity of 53.89 μA mM^-1 cm^-2 for glucose detection with an excellent stability and reproducibility. The excellent performance of the biosensor is ascribed to the stable three-dimensional structure of the composite, where graphene sheets provide a large surface area for immobilizing the two enzymes and CNTs inserted between the graphene sheets to decrease electron transfer resistance.
- Graphene,
- Ionic liquid,
- Biosensor,
- Electrochemistry

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References

Alwarappan S, Liu C, Kumar A, et al. Enzyme-doped graphene nanosheets for enhanced glucose biosensing[J]. J Phys Chem C, 2010, 114(30):12920-12924.

Kang X, Wang J, Wu H, et al. Glucose oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing[J]. Biosensors and Bioelectronics, 2009, 25(4):901-905.

Li F, Wang Z, Shan C, et al. Preparation of gold nanoparticles/functionalized multiwalled carbon nanotube nanocomposites and its glucose biosensing application[J]. Biosensors & Bioelectronics, 2009, 24(6):1765-1770.

Lin Y, Lu F, Tu Y, et al. Glucose biosensors based on carbon nanotube nanoelectrode ensembles[J]. Nano Letters, 2003, 4(2):191-195.

Wooten M, Karra S, Zhang M, et al. On the direct electron transfer, sensing, and enzyme activity in the glucose oxidase/carbon nanotubes system.[J]. Analytical Chemistry, 2014, 86(1):752-757.

Xu Q, Gu S X, Jin L, et al. Graphene/polyaniline/gold nanoparticles nanocomposite for the direct electron transfer of glucose oxidase and glucose biosensing[J]. Sensors & Actuators B Chemical, 2014, 190(1):562-569.

Wang J. Electrochemical glucose biosensors.[J]. Chemical Reviews, 2008, 108(2):814-825.

Jia W, Guo M, Zheng Z, et al. Electrocatalytic oxidation and reduction of H₂O₂ on vertically aligned Co₃O₄ nanowalls electrode:Toward H₂O₂ detection[J]. Journal of Electroanalytical Chemistry, 2009, 625(1):27-32.

Salimi A, Compton R G, Hallaj R. Glucose biosensor prepared by glucose oxidase encapsulated sol-gel and carbon-nanotube-modified basal plane pyrolytic graphite electrode[J]. Analytical Biochemistry, 2004, 333(1):49-56.

Sassolas A, Blum L J, Leca-Bouvier B D. Immobilization strategies to develop enzymatic biosensors[J]. Biotechnology Advances, 2012, 30(3):489-511.

Tran D N, Balkus K J. Perspective of Recent Progress in Immobilization of Enzymes[J]. Acs Catalysis, 2011, 1(8):956-968.

Wan L C, Feng W, Guo X, et al. Identification and characterization of small non-coding RNAs from Chinese fir by high throughput sequencing[J]. Bmc Plant Biology, 2012, 12(11):1-15.

Wu H, Wang J, Kang X, et al. Glucose biosensor based on immobilization of glucose oxidase in platinum nanoparticles/graphene/chitosan nanocomposite film. Talanta[J]. Talanta, 2009, 80(1):403-406.

Zargoosh K, Chaichi M J, Shamsipur M, et al. Highly sensitive glucose biosensor based on the effective immobilization of glucose oxidase/carbon-nanotube and gold nanoparticle in nafion film and peroxyoxalate chemiluminescence reaction of a new fluorophore.[J]. Talanta, 2012, 93(2):37-43.

Zhang J, Zhang F, Yang H, et al. Graphene oxide as a matrix for enzyme immobilization[J]. Langmuir, 2010, 26(9):6083-6085.

Rahman M M, Umar A, Sawada K. Development of amperometric glucose biosensor based on glucose oxidase co-immobilized with multi-walled carbon nanotubes at low potential[J]. Sensors & Actuators B Chemical, 2009, 137(1):327-333.

Chen X H Y, Gs. W. Glucose microbiosensor based on alumina sol-gel matrix/electropolymerized composite membrane[J]. Biosensors & Bioelectronics, 2002, 17(11-12):1005-1013.

Yujun Song, Konggang Qu, Chao Zhao, et al. Graphene oxide:Intrinsic peroxidase catalytic activity and its application to glucose detection[J]. Advanced Materials, 2010, 22(19):2206-2210.

Niu J, Lee J Y. Renewable-surface graphite-ceramic enzyme sensors for the determination of hypoxanthine in fish meat[J]. Analytical Communications, 1999, 36(3):81-83.

Stankovich S, Dikin D A, Piner R D, et al. Synthesis of graphene-based nanosheets via chemical reduction of exfoliated graphite oxide[J]. Carbon, 2007, 45(7):1558-1565.

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