The mechanism of the fluorescence quenching of L-tryptophan by graphene oxide

GONG Qiao-juan; WANG Yong-dong; YANG Hai-ying; YUAN Mei-ni

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Article Navigation > New Carbon Mater. > 2016 > 31(6): 639-645

GONG Qiao-juan, WANG Yong-dong, YANG Hai-ying, YUAN Mei-ni. The mechanism of the fluorescence quenching of L-tryptophan by graphene oxide. New Carbon Mater., 2016, 31(6): 639-645.

Citation:

GONG Qiao-juan, WANG Yong-dong, YANG Hai-ying, YUAN Mei-ni. The mechanism of the fluorescence quenching of L-tryptophan by graphene oxide. New Carbon Mater., 2016, 31(6): 639-645.

GONG Qiao-juan, WANG Yong-dong, YANG Hai-ying, YUAN Mei-ni. The mechanism of the fluorescence quenching of L-tryptophan by graphene oxide. New Carbon Mater., 2016, 31(6): 639-645.

Citation:

GONG Qiao-juan, WANG Yong-dong, YANG Hai-ying, YUAN Mei-ni. The mechanism of the fluorescence quenching of L-tryptophan by graphene oxide. New Carbon Mater., 2016, 31(6): 639-645.

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The mechanism of the fluorescence quenching of L-tryptophan by graphene oxide

1.
Department of Applied Chemistry, YunCheng University, YunCheng 044000, China;
2.
College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;
3.
School of Mechatronics of Engineering, North University of China, Taiyuan 030051, China

Funds: Financial support from the Science Foundation of "131" Leading talents project of Shanxi Province.; Science Foundation of Shanxi (2012011007-1); Science Foundation of Bureau of Science and Technology of Yuncheng(2060499).

Received Date: 2016-06-27
Accepted Date: 2016-12-26
Rev Recd Date: 2016-10-24
Publish Date: 2016-12-28

Abstract

Abstract

Graphene oxide (GO) was prepared by the modified Hummers method and used as a fluorescence quenching reagent of L-tryptophan (L-Trp). The structure of GO was characterized by FT-IR and Raman spectroscopy. The binding mechanism of L-Trp on GO and their reaction were investigated by UV spectroscopy and the fluorescence method. Results show that GO has a strong fluorescence quenching ability for L-Trp. The quenching efficiency is 95.6% for L-Trp with a concentration of 2×10^-5 mol·L^-1 when the GO concentration is 16×10^-5 g·mL^-1. The binding constants of L-Trp on GO were 2.03×10⁵, 1.83×10⁵ and 1.63×10⁵ mL·g^-1 at 25, 30 and 35℃, respectively, as revealed by the Lineweawer-Burk equation, indicating a strong interaction between GO and L-Trp. GO quenches the intrinsic fluorescence of L-Trp by the formation of a complex, and is a static quenching mechanism. The quenching rate constant was 6.65×10¹² mL·(g·s)^-1 at 25℃ as determined by the Stern-Volmer equation.
- Graphene oxide,
- L-tryptophan,
- Fluorescence quench

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References(22)

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