Preparation and biological properties of a graphene oxide/silk fibroin barrier membrane loaded with simvastatin

ZHAO Bin; WU Feng; BAI Ying-ying; FANG Min; WANG Lu

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Article Navigation > New Carbon Mater. > 2018 > 33(5): 460-468

ZHAO Bin, WU Feng, BAI Ying-ying, FANG Min, WANG Lu. Preparation and biological properties of a graphene oxide/silk fibroin barrier membrane loaded with simvastatin. New Carbon Mater., 2018, 33(5): 460-468.

Citation:

ZHAO Bin, WU Feng, BAI Ying-ying, FANG Min, WANG Lu. Preparation and biological properties of a graphene oxide/silk fibroin barrier membrane loaded with simvastatin. New Carbon Mater., 2018, 33(5): 460-468.

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Preparation and biological properties of a graphene oxide/silk fibroin barrier membrane loaded with simvastatin

1.
Shanxi Medical University Stomatological Hospital, Taiyuan 030001, China;
2.
Fenyang College of Shanxi Medical University, Fenyang 032200, China

Funds: Shanxi Applied Basic Research Program Science (201701D221065); Startup Foundation for Doctors of Shanxi Medical University (BS03201638).

Received Date: 2018-08-01
Accepted Date: 2018-11-01
Rev Recd Date: 2018-09-30
Publish Date: 2018-10-28

Abstract

Abstract

A graphene oxide/silk fibroin (GO/SF) barrier membrane loaded with simvastatin (SIM) was prepared by a freeze-drying method. The biocompatibility of the barrier membrane in vitro and its applicability in guided bone regeneration were investigated. The morphology,structure and the drug release behavior of the membrane were characterized by SEM, XRD and UV-spectroscopy. Laser confocal microscopy and CCK-8 tests were used to evaluate the attachment and proliferation of MC3T3-E1 dermal cells. Defective skulls of Sprague Dawley rats were covered with four membranes (GO/SF/SIM membrane, GO/SF membrane, SF/SIM membrane and SF membrane) and their effectiveness for bone regeneration was evaluated. Results indicated that the GO/SF/SIM membrane had a double-layer (dense surface and porous inside) and stable silk Ⅱ structure. SIM was continuously released from the barrier membrane over 15 days. The GO/SF membrane supported the attachment, growth and proliferation of MC3T3-E1 cells. The GO/SF/SIM exhibited the best bone repairing ability among the four membranes and a better biocompatibility in vitro than the GO/SF. The local application of GO had no damage to important organs (liver, spleen and kidney) of the rats in the short-term observation.
- Graphene oxide,
- Silk fibroin,
- Simvastatin,
- Guided bone regeneration,
- Barrier membrane

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References(27)

References

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