The effects of multi-wall carbon nanotubes on the toxicity and the expression of metabolic enzymes (GSTP1 and CYP1A1) in human liver cancer HepG2 cells

ZHAO Zhuo; LIU Ming; YANG Lei; LIU Wei; WANG Hua; LIU Mei-ling; ZHANG Xiu-xia; SHEN Jing; ZHANG Yuan

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Article Navigation > New Carbon Mater. > 2019 > 34(6): 600-605

ZHAO Zhuo, LIU Ming, YANG Lei, LIU Wei, WANG Hua, LIU Mei-ling, ZHANG Xiu-xia, SHEN Jing, ZHANG Yuan. The effects of multi-wall carbon nanotubes on the toxicity and the expression of metabolic enzymes (GSTP1 and CYP1A1) in human liver cancer HepG2 cells. New Carbon Mater., 2019, 34(6): 600-605.

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The effects of multi-wall carbon nanotubes on the toxicity and the expression of metabolic enzymes (GSTP1 and CYP1A1) in human liver cancer HepG2 cells

1.
Tianjin Customs Technical Center for Safety of Industrial Products, Tianjin 300308, China;
2.
College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China

Funds: General Administration of Customs Science and Technology Project (2018IK004).

Received Date: 2019-09-29
Accepted Date: 2020-01-03
Rev Recd Date: 2019-12-03
Publish Date: 2019-12-28

Abstract

Abstract

To investigate the toxic effect of multiwall carbon nanotubes (MWCNTs) on the human hepatoma cell line HepG2 and to determine whether MWCNTs may affect the expression of metabolic enzymes GSTP1 and CYP1A1in HepG2, HepG2 cells were treated with 0.12, 0.25, 0.5, 1 and 2 mg/mL MWCNTs for 24, 48 and 72 h, and the effect of MWCNTs on the proliferation of HepG2 cells was then measured by thiazolyl blue colorimetry. Real-time cell analysis (RTCA), which accurately monitored the inhibitory effect on the proliferation of breast cancer MCF-7 cells with MWCNT treatment and also avoided interference of MWCNTs with the data, was used to monitor the proliferation of HepG2 cells treated with different doses of MWCNTs (0.25, 0.5, 1 mg/mL). After 48 h of treatment with MWCNTs, the mRNA and protein level of metabolic enzymes in liver were detected by a real-time polymerase chain reaction and western blotting. Results indicated that MWCNTs inhibited the proliferation of HepG2 cells in a dose and time dependent manner. The mRNA level and protein level of the metabolic enzyme GSTP1 in HepG2 increased while the mRNA level and protein level of CYP1A1 in HepG2 decreased in the cells treated by MWCNTs compared with the control group.
- Multiwalled carbon nanotube,
- HepG2 cells,
- Toxicity study,
- Real-time cell analysis,
- Liver metabolic enzyme

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