Abstract: Graphene oxide (GO), as a derivative of two-dimensional graphene, has the characteristics of simple preparation, low cost, abundant functional groups and easy modification. The introduction of wrinkles in the two-dimensional GO nanosheets changes the morphology and structure, and thus can give GO special physical, chemical, biological and other characteristics, making it possible for use in many fields. Research progress on the preparation and applications of wrinkled GO (WGO) in recent years is reviewed, focusing on the preparation principles and characteristics of WGO, including pre-stretching, the solvent induction method, rapid drying, magnetic field induction, surfactant templating, electrophoretic deposition and pH adjustment methods. The applications of WGO in smart devices, biomedicine and water treatment are summarized. Current challenges in the preparation and use of WGO are discussed, and future development prospects are given.