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摘要: 由于红光/近红外发射具有深层组织穿透力强、自体荧光小、对生物组织损伤小等特点，具有上述特性的碳点的制备与生物成像应用备受关注。本文以磺化四苯基卟啉为前驱体，采用溶剂热法合成近红外发射的荧光碳点（NIR-CDs）。NIR-CDs的最大发射峰位于692 nm，其荧光发射具有激发波长非依赖性，经分析NIR-CDs的近红外荧光发射主要源于分子态发光。此外，NIR-CDs还具有良好的水溶性和生物相容性、丰富的表面官能团、低毒性和优异的细胞标记能力，证实了NIR-CDs在细胞近红外成像中的应用潜力。本研究有望促进面向生物应用的近红外荧光碳点的发展，推动新型碳点的研究与实际应用。Abstract: It is very difficult to prepare red/near-infrared emission carbon dots (CDs) for bio-imaging applications which are needed because of their deep tissue penetration, minimal auto-fluorescence, and low emission light damage to bio-tissues. Near-infrared emitting CDs (NIR-CDs) were synthesized from sulfonated tetraphenylporphyrin using a solvothermal method. They have excitation-independent properties with a maximum emission at 692 nm. Studies showed that this unique near-infrared emission mainly originated from the aggregated molecular states of the CDs. The NIR-CDs showed good water solubility, exceptional biocompatibility, low toxicity, and superior cellular labelling ability. This work could significantly advance the structural design and preparation of NIR-CDs and corresponding bio-imaging applications.
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Key words:
- Near-infrared emission /
- Molecular states /
- Cellular labelling /
- Bio-imaging
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Figure 2. (a) TEM and (b) the size distribution histogram of the NIR-CDs; (c, d) HRTEM images of the NIR-CDs. The white circles stand for crystalline NIR-CDs, and yellow circles represent amorphous CDs.
Figure 6. (a) Cell viability of HeLa cells after incubation with various concentrations of NIR-CDs; (b) Confocal images of Hela cells incubated with NIR-CDs at the concentration of 200 µg mL−1 obtained under dark field (right image), bright field (middle image) and their merged (left) image at the excitation of 543 nm laser.
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