Abstract: Graphite oxide was synthesized by the Hummer method, followed by ultrasonic delamination in water to produce a stable hydrosol of graphene oxide sheets. Then, a flowdirected assembly of this graphene oxide was produced in a celluloseacetatebased microfilter to form an ordered nanostructured membrane. The membrane was treated by chemical reduction and carbonization for deoxidation and regraphitization, which increased its electrical conductivity while retaining its shape. The graphenebased conductive membrane had a conductivity of 184.8S/cm. A reductive treatment before the carbonization increased the mass of residual carbon in the resulting membranes.