聚苯胺/氧化石墨的合成及其在DNA识别上的应用

Polyaniline-intercalated graphite oxide (PAn/GO) composites were synthesized by a procedure similar to an exfoliation/adsorption followed by in situ polymerization. The microstructure and electrochemical activity of PAn/GO composite were investigated by TEM, AFM, XRD and FTIR. Calf thymus single-stranded DNA (CTssDNA) and double-stranded DNA (CTdsDNA) were indentified by square wave voltammograms (SWV) using PAn/GO composite modified carbon paste as a working electrode. Results show that the PAn/GO composite in an ellipsoidal shape and with AN equivalent diameter of 60-70nm, aggregates to form chains and possesses electrochemical activity. A portion of PAn was intercalated into GO by two parallel chains, and the other portion was coated on the surfaces of the GO. The SWV redox potentials of a PAn/GO composite modified carbon paste electrode in CTssDNA and CTdsDNA solutions are 90.99mV and 18.00mV respectively.